This term is used to identify and describe interaction data displayed at SGD. In this type of experiment, an interaction is detected by chromatographic purification (for example, GST fusions purified with glutathione-Sepharose beads).

A method of separating biochemical components based on specific interactions between the components of the biochemical mixture and other molecules (e.g., antigen-antibody or receptor-ligand).

A method for separating molecules by exploiting their ability to bind specifically to other molecules. There are several types of biological affinity chromatography. A biological molecule can be immob [..]

A column chromatographic technique that employs attached functional groups that have a specific affinity for sites on particular proteins.

(French : chromatographie d’affinité) Any form of chromatography in which the components of the sample are separated on the basis of chemical affinity for a substance such as a binding protein or an i [..]

A chromatographic technique that utilizes the ability of biological molecules to bind to certain Ligands specifically and reversibly. It is used in protein Biochemistry. (McGraw-Hill Dictionary of Sci [..]

A chromatographic method used to isolate biomolecules that reversibly bind a molecule attached to a solid-phase support.

<investigation> A technique of analytical chemistry used to separate and purify a biological molecule from a mixture, based on the attraction of the molecule of interest to a particular ligand which has been previously attached to a solid, inert substance. The mixture is passed through a column containing the ligand attached to the stationary [..]